LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(2183..2279)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(2294..2313)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2321..2337
                     /note="M13 Reverse priming site"
     gene            2450..3259
                     /note="Kanamycin resistance gene"
     rep_origin      3380..4053
                     /note="pUC origin"
     vector          join(2191..4056,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..2182
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:177995 IMAGE:9052978"
                     /tissue_type="Pooled, cerebellum, kidney, placenta,
                     testis, lung, colon, liver, heart, thyroid, bladder,
                     uterus, PCR rescued clones"
                     /clone_lib="NIH_MGC_362"
                     /lab_host="DH10B"
                     /note="Vector: pCR4-TOPO with reversed insert; Clone
                     identification sequence tag: TCTGGATT"
     gene            692..2182
                     /gene="AMY1A"
                     /gene_synonym=AMY1B
                     /db_xref="GeneID:276"
                     /db_xref="HGNC:474"
                     /db_xref="MIM:104700"
     CDS             692..2182
                     /dnas_title="amylase, alpha 1A (salivary)"
                     /gene="AMY1A"
                     /gene_synonym=AMY1B
                     /codon_start=1
                     /product="amylase, alpha 1A (salivary)"
                     /protein_id="AAI44453.1"
                     /db_xref="GI:219518279"
                     /db_xref="GeneID:276"
                     /db_xref="HGNC:474"
                     /db_xref="MIM:104700"
                     /translation="MKLFWLLFTIGFCWAQYSSNTQQGRTSIVHLFEWRWVDIALECE
                     RYLAPKGFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCN
                     NVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGS
                     GDIENYNDATQVRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVAGFRIDASKHMW
                     PGDIKGILDKLHNLNSNWFPEGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAK
                     LGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDAR
                     LYKMAVGFMLAHPYGFTRVMSSYRWPRYFENGKDVNDWVGPPNDNGVTKEVTINPDTT
                     CGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWT
                     FSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAE
                     SKL"
     misc_feature    692..2182
                     /note="AMY1 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg ccagtattcc tcaaatacac aacaaggacg
        721 aacatctatt gttcatctgt ttgaatggcg atgggttgat attgctcttg aatgtgagcg
        781 atatttagct cccaagggat ttggaggggt tcaggtctct ccaccaaatg aaaatgttgc
        841 cattcacaac cctttcagac cttggtggga aagataccaa ccagttagct ataaattatg
        901 cacaagatct ggaaatgaag atgaatttag aaacatggtg actagatgca acaatgttgg
        961 ggttcgtatt tatgtggatg ctgtaattaa tcatatgtgt ggtaatgctg tgagtgcagg
       1021 aacaagcagt acctgtggaa gttacttcaa ccctggaagt agggactttc cagcagtccc
       1081 atattctgga tgggatttta atgatggtaa atgtaaaact ggaagtggag atatcgagaa
       1141 ctataatgat gctactcagg tcagagattg tcgtctgtct ggtcttctcg atcttgcact
       1201 ggggaaggat tatgtgcgtt ctaagattgc cgaatatatg aaccatctca ttgacattgg
       1261 tgttgcaggg ttcagaattg atgcttccaa gcacatgtgg cctggagaca taaaaggcat
       1321 tttggacaaa ctgcataatc taaacagtaa ctggttcccg gaaggtagta aacctttcat
       1381 ttaccaggag gtaattgatc tgggtggtga gccaattaaa agcagtgact actttggtaa
       1441 tggccgggtg acagaattca agtatggtgc aaaactcggc acagttattc gcaagtggaa
       1501 tggagagaag atgtcttact taaagaactg gggagaaggt tggggtttca tgccttctga
       1561 cagagcgctt gtctttgtgg ataaccatga caatcaacga ggacatggcg ctggaggagc
       1621 ctctatactt accttctggg atgctaggct gtacaaaatg gcagttggat ttatgcttgc
       1681 tcatccttat ggatttacac gagtaatgtc aagctaccgt tggccaagat attttgaaaa
       1741 tggaaaagat gttaatgatt gggttgggcc accaaatgat aatggagtaa ctaaagaagt
       1801 tactattaat ccagacacta cttgtggcaa tgactgggtc tgtgaacatc gatggcgcca
       1861 aataaggaac atggttaatt tccgcaatgt agtggatggc cagcctttta caaactggta
       1921 tgataatggg agcaaccaag tggcttttgg gagaggaaac agaggattca ttgttttcaa
       1981 caatgatgac tggacatttt ctttaacttt gcaaactggt cttcctgctg gcacatactg
       2041 tgatgtcatt tctggagata aaattaatgg caactgcaca ggcattaaaa tctacgtttc
       2101 tgatgatggc aaagctcatt tttctattag taactctgct gaagatccat ttattgcaat
       2161 tcatgctgaa tctaaattgt aaCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA
       2221 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG
       2281 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA
       2341 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC
       2401 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT
       2461 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA
       2521 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA
       2581 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC
       2641 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA
       2701 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC
       2761 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT
       2821 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT
       2881 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT
       2941 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT
       3001 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT
       3061 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA
       3121 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG
       3181 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT
       3241 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC
       3301 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC
       3361 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
       3421 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
       3481 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
       3541 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
       3601 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
       3661 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
       3721 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
       3781 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
       3841 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
       3901 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
       3961 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
       4021 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT
//
